Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy
نویسندگان
چکیده
Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site diagnostics with a cost-effective mobile-phone-based multimodal microscope. We demonstrate that targeted next-generation DNA sequencing reactions and in situ point mutation detection assays in preserved tumour samples can be imaged and analysed using mobile phone microscopy, achieving a new milestone for tele-medicine technologies.
منابع مشابه
Studies on Genotoxic Effects of Mobile Phone Radiation on A375 Cells
Introduction: Radiation from cell phones has been associated with an increased risk of cancer. The literature has reported evidence of certain biological effects resulting from exposure to various wavelengths, doses, and intensities of radiofrequency radiation. The present study aimed to evaluate the possible adverse effects of radiation from a GSM mobile phone operating at 900 MHz on human mel...
متن کاملI-37: Establishing High Resolution Genomic Profiles of Single Cells Using Microarray and Next-Generation Sequencing Technologies
The nature and pace of genome mutation is largely unknown. Standard methods to investigate DNA-mutation rely on arraying or sequencing DNA from a population of cells, hence the genetic composition of individual cells is lost and de novo mutation in cell(s) is concealed within the bulk signal. We developed methods based on (SNP-) arraying and next-generation sequencing of single-cell whole-genom...
متن کاملEvaluation of BRAF-V600E gene mutation in colon tissue of patients with colorectal cancer in Iran
Background: Colorectal cancer is one of the most common types of cancer and the cause of death of a large number of patients and requires investigating the causes of the disease and adopting targeted therapies. Considering the diagnostic, therapeutic, and prognostic significance of genetic markers, in the present study BRAF-V600E gene mutation was evaluated in tissue samples of colorectal cance...
متن کاملIdentification of a Novel Arylsulfatase B Gene Mutation in Three Unrelated Iranian Mucopolysaccharidosis Type-VI Patients with Different Phenotype Severity
Background: Mucopolysaccharidosis type-VI (MPS-VI), which is inherited as an autosomal recessive trait, results from the deficiency of N-acetylgalactosamine 4-sulfatase (arylsulfatase B) activity and the lysosomal accumulation of dermatan sulfate. In this study, ARSB mutation analysis was performed on three unrelated patients who were originally from the West Azerbaijan province of Iran. Method...
متن کاملInvestigating the relationship between mobile phone harmful use and Internet addiction with emotional divorce of teachers in Kermanshah city
The purpose of this study was to investigate the relationship between mobile phone harmful use and internet addiction with the emotional divorce of female teachers in Kermanshah. The statistical population of this study included all married female teachers in Kermanshah city in 1969-97. Using multi-stage cluster sampling, 269 people were selected as sample based on Morgan's table. The questionn...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 8 شماره
صفحات -
تاریخ انتشار 2017